| المستخلص: |
The present study is designed to isolated and identification the pathogenic bacteria associated with Cutaneous leishmaniasis. Cutaneous leishmaniasis (CL) is a parasitic disease characterized by single or numerous ulcerations. This was study performed in Iraqi in Shatra general hospital and Al-hussein teaching hospital in Thi-Qar province 2016- 2017. Total of 370 samples of suspected patients were taken and after examining them using geimsa stain, the positive were 186 males and 126 females then sterilize the area surrounding for ulcer, all samples were taken to (swab transport media), culture to MacConkey agar and blood agar after 24 hr of incubation at 37C, after diagnosed by biochemical testing then species determined by API system. The positive results on culture media 92/312, males 62 (67.4%), female 30 (32.6%). The total bacteria isolated from the lesions were in males and female staph auerus 52 cases (56.5%), Escherichia coli 8 cases (8.6%), Bacillus cereus 6 cases (6.5%), Pseudomonas. aeruginesa 5 cases (5.4%), Strep. Pyogenes 6 cases (6.5%), Strepto. agalactiae 3 cases (3.3%).While other that isolated in males only and did not appear in females were aerobic bacteria, Listeria moncytogenes 3 cases (3.4%), Actinobacteria spp 2 cases(2.2%), Klebsiella. Pneumonia 2 cases (2.2%), Rodococcus .equi 1 cases (1.1%), and anaeroberia bacteria, Peptococcus niger 2 cases (2.2%), Peptostreptococcus. spp 2 cases (2.2%). Then find bacteria that more presence more with cutaneous Leishmaniasis, were it was staph. aureus therefore it were objected study antibiotic resistant. The present study demonstrates that Staph. Aureus isolates were resistant to methicillin 50 (96.15%), penicillin 48 (92.30%), Clindamycin 37 (71.15%), ciprofloxacin 30 (57.69%), Gentamicin 30 (57.69%), Amoxicalave 29 (55.76%), Azithromycin 25 (48.07%), Amoxicillin 22 (42.30%), Tetracycline 21 (40.38%), Rifampin 10 (19.23%) and Vancomycin all staph .auerus are sensitive to Vancomycin The all 52 Staph. aureus isolates were further examined using polymerase chain reaction (PCR) for detection of (16SrRNA, mecA and PVI gene). 16SrRNA gene which were responsible for diagnosis, where all isolates were positive to was 52* (100%), mecA gene which were responsible for methicillin resistance this was detected in 52* (100%). of Staph. aureus isolates and a part of virulence factors PVL gene was detected in 46/52(88.46%) of Staph. aureus isolates which are responsible to kill the leukocytes of human and other animals.
|
