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|a eng
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|9 552245
|a Almagatef, Asma Basheir
|e Author
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|a The Prevalence of CTX-M-15 among Escherichia coli Isolates Associated with Urinary Tract Infection in Tripoli
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|a معدل انتشار بكتيريا 15 - CTX -M من نوع الإيشيريشيا القولونية المعزولة من المرضى المصابين بمرض التهاب المسالك البولية في طرابلس
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| 260 |
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|a طرابلس
|c 2017
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| 300 |
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|a 1 - 92
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| 336 |
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|a رسائل جامعية
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| 502 |
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|b رسالة ماجستير
|c جامعة طرابلس
|f كلية الصيدلة
|g ليبيا
|o 0015
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| 520 |
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|a Urinary tract infections (UTIs) are among the most common bacterial infections encountered in clinical practice and account for significant morbidity and high medical costs. Most UTIs are caused by of the family Enterobacteriaceae mainly Escherichia coli and Klebsiella spp. High resistance rates to antimicrobials used in the treatment of UTIs among E. coli and other uropathogens have been reported worldwide, particularly in developing countries including Libya. The problem worst by emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among E. coli organism in recent years. However, there is little information on the detection of ESBLs genes in E. coli from patients with UTIs in the Arab countries using polymerase chain reaction (PCR) and in Libya such information is lacking. This study aimed to determine the prevalence of ESBLs and to detect CTX-M-15 gene among E. coli clinical isolates obtained from Tripoli hospitals. A total of 346 (222 female, 124 male) clinical E. coli isolates collected during 12 months from January 2013 to February 2014 from urine clinical specimens obtained from five teaching hospitals in Tripoli: Tripoli Medical Centre TMC; Tripoli Paediatric Hospital (TPH): Khadra Hospital; Aljala Hospital and Tripoli Central Hospital (TCH). The distribution of ESBL-producing E. coli varied among the different hospitals with the highest proportion in TMC (67.6%) compared with other hospitals: TPH (9.9%), Khadra hospital (9.9%), Aljala hospital (8.4%) and TCH (4.2%). Females provided 51/71 (71.8%) of the isolates expressing ESBL compared with male patient 20/71 (28.2%). According to antimicrobial susceptibility tests for ESBL producing 71/346 (20.5%) strains and non ESBL producers 275/346 (79.5%) there was variable degree of resistance against commonly used antibiotics. Extremely high resistance rates were observed to ceftriaxone, cefepime, and ceftazidime (93%-100%) among ESBL producer compared to non-ESBL producer (2.2-4.7%). High rates of resistance were also demonstrated to ciprofloxacin (67.7%) and cefotoxim (64.8%), and lower levels of resistance (2.8%) were exhibited to amikacin, meropenem and etrapenem. Overall MDR were detected in 22.2% of isolates. The majority of ESBL-positive E. coli was positive for CTX-M-15 gene by PCR (61/71) (85.9%). This study highlights the high prevalence of ESBL production among E. coli in clinical setting in Libya. There is an urgent need for surveillance studies on antimicrobial resistance and prevalence of ESBLs among uropathogens to guide the clinical treatment of UTIs in Libya in the future.
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|a طرابلس
|a المسالك البولية
|a بكتيريا 15 - CTX -M
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| 700 |
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|9 433685
|a Sufya, Najib M.
|e Advisor
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| 700 |
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|9 552239
|a Zorgani, Abdulaziz A.
|e Advisor
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| 856 |
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|u 9814-005-010-0015-T.pdf
|y صفحة العنوان
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| 856 |
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|u 9814-005-010-0015-A.pdf
|y المستخلص
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| 856 |
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|u 9814-005-010-0015-C.pdf
|y قائمة المحتويات
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| 856 |
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|u 9814-005-010-0015-F.pdf
|y 24 صفحة الأولى
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| 856 |
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|u 9814-005-010-0015-1.pdf
|y 1 الفصل
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| 856 |
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|u 9814-005-010-0015-2.pdf
|y 2 الفصل
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| 856 |
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|u 9814-005-010-0015-3.pdf
|y 3 الفصل
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| 856 |
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|u 9814-005-010-0015-4.pdf
|y 4 الفصل
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| 856 |
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|u 9814-005-010-0015-O.pdf
|y الخاتمة
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| 856 |
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|u 9814-005-010-0015-R.pdf
|y المصادر والمراجع
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| 930 |
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|d y
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| 995 |
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|a +Dissertations
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| 999 |
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|c 1019413
|d 1019413
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