المستخلص: |
The physiological process of reproduction is an important aspect in the bivalve species life. The Pacific oyster, Crassostrea gigas, is considered one of the most important marine organisms for both commercial and ecological purposes. Pacific oyster is also inexpensive and easy to produce because it does not require extra nourishment to sustain its growth. The herpes virus, OsHV-1, is thought to be one of the most serious causes of diseases that affect the productivity of oysters. However, it is possible to improve and develop the genital glands and sperm of oysters through the use of additives (for example, Selenium). The objective of this study was to investigate the impact of selenium on the spermatozoa of C. gigas. In order to achieve this goal, the study developed a method to house oysters and examine their health and sperm. In the first experiment, designed to determine the health of animals being used in the experiment, oyster samples from both Galway Bay and Carlingford Lough were processed to check for the presence of Herpes virus. This was done using DNA extraction and PCR. The experiment was concluded following the emergence of algae in seawater tanks and an increase in mortality seen. Experiment 2 began by setting up tanks which were filled with seawater. Following this, different doses of selenium were added and the number, vitality and motility of sperm was examined. No significant impact was observed by the variables (condition, tank and weight) on the number, vitality, and motility of the sperm. Furthermore, there was no significant correlation between the number of sperm and the weight of oyesters, although a significant correlation was recorded between weight and length of the oysters. This research has shed light on the impact, or lack thereof, of selenium on oyster sperm.
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