Association between Nuclear Factor Kappa B Gene Polymorphism "rs28362491" and Interleukin 10 in Jordanian Rheumatoid Arthritis Patients
| العنوان بلغة أخرى: |
العلاقة بين تعدد الشكل الجيني للعامل النووي كابا ب "rs28362491" وانترلوكين 10 في مرضى التهاب المفاصل الروماتويدي الأردنيين |
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| المؤلف الرئيسي: | الزيود، صفاء (مؤلف) |
| مؤلفين آخرين: | القيسي، طلال سالم (مشرف) , القرالة، معاذ ياسين عبدالله (مشرف) |
| التاريخ الميلادي: |
2021
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| موقع: | السلط |
| الصفحات: | 1 - 75 |
| رقم MD: | 1210679 |
| نوع المحتوى: | رسائل جامعية |
| اللغة: | الإنجليزية |
| الدرجة العلمية: | رسالة ماجستير |
| الجامعة: | جامعة عمان الأهلية |
| الكلية: | عمادة الدراسات العليا والبحث العلمي |
| الدولة: | الاردن |
| قواعد المعلومات: | Dissertations |
| مواضيع: | |
| رابط المحتوى: |
عدد مرات التحميل
1
| المستخلص: |
Rheumatoid arthritis (RA) is a common chronic systemic autoimmune disease. Cytokines such as Interleukin-10 (IL-10), Interleukin-6 (IL-6) and Tumor Necrotic Factor-(TNF-) play important roles in the pathophysiology of RA. Nuclear factor Kappa B (NF-κB) is one of the important transcription factors that is activated during both early and late stages of inflammation. Therefore, the aim of this study was to evaluate the effect of different variation of NF-κB polymorphism (rs28362491) on IL-10 expression level in RA patients in Jordanian population. Methods: Seventy-four blood samples were divided into two groups which are: control group (n=24) and the RA patient group (n=50). Two blood sample were taken from each participant. One for NF-κB polymorphisms (rs28362491) genotyping assay by real-time PCR using TaqMan SNP Genotyping Assays, and the other for measuring the concentration of the human IL-10 using ELISA test. xiv Results: The IL-10, Anti -CCP, CRP, RF, and ESR were significantly increased in RA patients when compared with the control group (P<0.01). However, there was no significant difference in the genotype and allele frequencies of -94 ATTG Ins/Ins, Ins/Del and Del/Del polymorphisms (rs28362491) between RA and control group. Additionally, IL-10 levels were significantly increased in RA patients that carry Del/Del alleles compare with the other alleles (302.0±46.9 pg/ml vs. 256.0.0±36.9 and 247.2±44.5 pg/ml, respectively). Furthermore, The Del/Del polymorphism had significantly higher (P<0.05) Anti-CCP levels than both Ins/Ins and Ins/Del polymorphisms Conclusion: Our findings showed that the Del/Del allele in the NFB1 –94ins/del ATTG polymorphism influences the severity and progression of RA in Jordanian patients through regulating IL-10 levels. |
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