Influence of GC Content of Primer on the Number of Polymorphic Bands in Phylogenetic Analysis of Uropathogenic Escherichia Coli
| المصدر: | المجلة الجامعة |
|---|---|
| الناشر: | جامعة الزاوية - مركز البحوث والدراسات العليا |
| المؤلف الرئيسي: | Alshrif, Naima M. (Author) |
| مؤلفين آخرين: | Buazzi, Mahmoud M (Co-Author) , Elgared, Sleman A. (Co-Author) |
| المجلد/العدد: | مج17, ع2 |
| محكمة: | نعم |
| الدولة: |
ليبيا |
| التاريخ الميلادي: |
2015
|
| الشهر: | أغسطس |
| الصفحات: | 55 - 72 |
| رقم MD: | 1263861 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | EduSearch, EcoLink, IslamicInfo, AraBase, HumanIndex |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
RAPD | Uropathogenic E. Coli | UTI | Libya
|
| رابط المحتوى: |
الناشر لهذه المادة لم يسمح بإتاحتها. |
| المستخلص: |
Fourteen serotypes of uropathogenic Escherichia coli, causing Urinary Tract Infection, were isolated from inpatients at Khoms University Hospital in Khoms, Libya, and subjected to biochemical, biogramatic, and phylogenetic analysis using fifteen random ten nucleotide primers. DNA amplification with each of the primers resulted in generation of different DNA fingerprinting profile with varied number of bands. Dendrogram based generation of clustering of E. coli serotypes showed three major clusters, members in one of which showed most close genetic profile, and biochemical and biogramatic properties as well. Size or G+C content of the arbitrary primers did not influence number of polymorohic bands, nor does it seem to be an essential requirement for the generation of informative RAPD profiles in tested strains. |
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