Evaluation of Genetic Diversity in Seven Yemeni Indigenous Sorghum Cultivars Based on Proteins, Isozymes and DAN Markers by Using SDS-PAGE, Native-PAEG and RAPD-PCR Techniques
| المصدر: | مجلة أبحاث البيئة والتنمية المستدامة |
|---|---|
| الناشر: | الرابطة العربية للعلوم البيئية وجامعة الناصر |
| المؤلف الرئيسي: | A., Tayeb A. I. (Author) |
| مؤلفين آخرين: | Al-Shabi, J. H. M. (Co-Author) , Al-Shebani, Y. A. (Co-Author) |
| المجلد/العدد: | مج10, ع2 |
| محكمة: | نعم |
| الدولة: |
اليمن |
| التاريخ الميلادي: |
2021
|
| الشهر: | يونيو |
| الصفحات: | 213 - 233 |
| ISSN: |
5132-5124 |
| رقم MD: | 1443874 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | EcoLink |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
Sorghum | Proteins | Isozymes | Α-Esterase | Peroxidase | DAN Markers | SDS-PAGE | Native-PAEG | RAPD-PCR
|
| رابط المحتوى: |
| المستخلص: |
This study was carried out in the objective to evaluate the genetic diversity in seven indigenous Sorghum cultivars collected from six different agro-climatic regions in Yemen, based on their biochemical characters (proteins, isozymes and DNA markers by using SDS-PAGE, Native PAEG and RAPD-PCR techniques). The banding pattern profiles of total soluble proteins markers, monitored that all studied cultivars revealed different banding profiles, with 36 bands distributed in all the cultivars. Out of which, 18 unique bands could be considered as a positive specific biochemical genetic markers for genetic diversity evaluation of these cultivars. Isozyme markers (α-Esterase and Peroxidase) profiles revealed three bands from each of the two isozymes were distributed in all sorghum cultivars out of which 1 band was unique band that observed in one cultivar (Zaaer for α-Esterase and Goahri for peroxidase). These bands could be considered as a positive specific biochemical genetic marker for genetic diversity evaluation of these cultivars. All the seven used random primers monitored different DNA polymorphism among all the seven sorghum cultivars and produced a total of 80 bands (amplicons) with an average of 11.43% bands per primer, 0.74 polymorphic banding patterns. The overall results showed that all the seven primers used in this study gave specific unique bands for each of the studied cultivars and could be considered as taxonomical markers. |
|---|---|
| ISSN: |
5132-5124 |
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