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|3 10.54633/2333-023-049-019
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| 041 |
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|a eng
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| 044 |
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|b العراق
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| 100 |
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|9 772734
|a Faisal, Samih Riyadh
|e Author
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| 245 |
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|a The Role of PknF and FbpA as a Virulence Genes with Interleukin4-and 6, in the Pathogenesis of Tuberculosis
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|b جامعة ميسان - كلية التربية الأساسية
|c 2024
|g آذار
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| 300 |
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|a 184 - 192
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| 336 |
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|a بحوث ومقالات
|b Article
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|b Tuberculosis (TB) is a global health problem caused by Mycobacterium tuberculosis (MTB), a bacterium that can evade the host immune system and keep at in a latent state. Drug-resistant strains of MTB, such as multi-drug resistant TB (MDR-TB), pose a significant challenge toward the TB control efforts. This study aimed to investigate the role of two MTB virulence genes, pknF and fbpA, and two host cytokines, Interleukin-4 and Interleukin-6, in the pathogenesis of TB. The expression levels of pknF and fbpA genes were measured by qPCR in (12) sensitive TB and (12) MDR-TB isolates. IL-4 and IL-6 were measured by ELISA in serum samples from (24) healthy controls, (12) patients having sensitive TB, and 12 patients having MDR-TB. The results revealed a significant difference in fbpA gene expression between MDR-TB (2.59 ± SE 0.36) and sensitive TB (1.01 ± SE 0.037) isolates (P = 0.0003), whereas pknF gene expression did not vary significantly across the two groups (2.53 ± 0.62 in MDR-TB and 1.72 ± 0.40 in sensitive TB) (p = 0.289). IL-4 levels were markedly elevated in patients with MDR-TB (1091.967 ± SE 108.793 pg/ml) compared to the control group (105.3358 ± SE 5.543 pg/ml) (p<0.0001), but not significantly different from patients with sensitive TB (1054.763 ± SE 71.482 pg/ml). IL-6 levels were significantly higher in both MDR-TB and sensitive TB patients than in the control group (9.253 ± SE 0.456 pg/ml). However, MDR-TB patients showed a non-significant lower ratio of IL-6 (38.5851 ± Se 4.601 pg/ml) than sensitive TB patients (42.458 ± SE 1.809). A significant negative correlation were observed between fbpA gene expression and IL-4 levels in both MDR-TB and sensitive TB patients (r = -0.375; p < 0.0001 and r = -0.165; p < 0.0001, respectively), and a positive correlation between fbpA gene expression and IL-6 levels in both groups (r = 0.1006; p < 0.0001 and r = 0.466; p < 0.0001, respectively). These findings suggest that pknF and fbpA genes may play a role in the virulence of MTB, especially in drug-resistant strains, and that IL-4 and IL-6 may be involved in the host immune response to MTB infection. These potential biomarkers could be used to develop targeted therapies for MDR-TB and improve TB control efforts globally.
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| 653 |
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|a مرض السل
|a المشكلات الصحية
|a البكتريا الضارة
|a الجهاز المناعي
|a الأدوية العلاجية
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| 692 |
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|b Tuberculosis
|b Mycobacterium Tuberculosis
|b PknF Gene
|b FbpA Gene
|b Interleukin 4
|b Interleukin 6
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| 700 |
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|9 772733
|a Alshammarii, Zahrah Adnan Dakhel
|e Co-Author
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| 773 |
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|4 التربية والتعليم
|4 العلوم الإنسانية ، متعددة التخصصات
|6 Education & Educational Research
|6 Humanities, Multidisciplinary
|c 019
|e Maisan Journal of Academic Studies
|f Maǧallaẗ Mīsān li-l-dirāsāt al-akādīmiyyaẗ
|l 049
|m مج23, ع49
|o 2333
|s مجلة ميسان للدراسات الأكاديمية
|v 023
|x 1994-697X
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| 856 |
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|u 2333-023-049-019.pdf
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| 930 |
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|d y
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|q n
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|a EduSearch
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| 995 |
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|a HumanIndex
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|c 1458265
|d 1458265
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