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الرئيسية > استخلاص انزيمات ألفا أميليز a-...>الوصف

استخلاص انزيمات ألفا أميليز a-amylase وبروتيز protease وزابلنيز Xylanase من بكتيريا bacillus subtilis والفطر Aspergillus niger وبيان تأثيرها في نمو الجرذان

العنوان بلغة أخرى: Extraction of α-Amylase, Protease and Xylanase Enzymes From Bacillus Subtilis and Aspergillus Niger and Estimations the Enzymes Effects on Rats
المؤلف الرئيسي: هجيج، جدوع محمد (مؤلف)
مؤلفين آخرين: السامرائي، عبدالمنعم حمد مجيد (مشرف) , الجبورى، كركز محمد ثلج عبدون (مشرف)
التاريخ الميلادي: 2013
موقع: تكريت
التاريخ الهجري: 1434
الصفحات: 1 - 120
رقم MD: 597232
نوع المحتوى: رسائل جامعية
اللغة: العربية
الدرجة العلمية: رسالة دكتوراه
الجامعة: جامعة تكريت
الكلية: كلية التربية
الدولة: العراق
قواعد المعلومات: Dissertations
مواضيع:
البكتيريا | الفطريات | المركبات الكيميائية | الأنزيمات | فئران التجارب
رابط المحتوى:
صفحة العنوان     
المستخلص     
قائمة المحتويات     
24 صفحة الأولى     

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المستخلص: This study was conducted in the laboratories of the Department of Food Science-College of Agriculture at Tikrit University, for a period from1st of Oct.2010 tothe last of Dec. 2011 forisolation and identification of Aspergillus nigerand Bacillus subtilismicrobes,capable to produce the α-amylase, Protease and Xylanase Enzymes and determine the optimal and the stability of each enzymes against different levels of pH and temperature, In addition to illustrate the effects of orally doses from each enzymes on the growth rates and some of natural and biochemical blood parameters of the growth rats after dosage for 21 days. The results were indicatedthateach microbe was capable to produce the α-amylase, protease and the xylenase enzymes after changed the production medium for each ones produced, especially tochange the induced ingredients. The optimal pH for each enzyme which produced from Aspergillus niger showed optimal activity at acidic rangesat approximately pH 5, while the enzymes produced from Bacillus subtiliswas appear optimal activityat alkalineranges at pH 8 to 9.The stability of enzymes wasappearingat the same conditions for production at each ones. The orally dosage of enzymesfor growth rats indicated that it has an effects on the amelioration of the growth rates and feed conversion ratio with each concentrations of enzymes. In additions, the orally dosages caused asignificant (p< 0.05) increased of white blood cells countand the percentage of Neutrophils were also increased while the lymphocytes were decreased significantly. The results indicated that the globulinsproteins were increased in proportion to the enzymes concentration orally dosages, while the total proteins and the albumins proteins were not significantly affected. The activity of serum enzymes AST, ALT and ALP appeared to be significantly decreased with the increased of orally dosages enzymes compared with same values of it's at rats of control group.

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