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|a eng
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| 100 |
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|9 12900
|a Al Homrani, Majid G.
|e Author
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| 245 |
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|a ISOLATION AND CHARACTERISATION OF HAEMATOPOIETIC STEM CELL
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| 260 |
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|a ملبورن
|c 2011
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| 300 |
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|a 1 - 30
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| 336 |
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|a رسائل جامعية
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| 502 |
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|b رسالة ماجستير
|c RMIT University
|f School of Medical Science
|g أستراليا
|o 0012
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| 520 |
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|a The heterogeneous population of haematopoietic stem cells (HSCs) in the mammalian bone marrow poses as a potential problem during their isolation for future transplantation. HSCs are the cells that have the capacity to generate all blood cells and are defined a bona fide stem cells since they convey the properties for self-renewal. Based on the fact that HSCs are present in dramatically low numbers in the bone marrow niche and that they don’t have a unique identifying feature or phenotype to detect them, these parameters increase the difficulty in their isolation. However, recent advances in cluster of differentiation (CD) marker identification on these cells, has enabled investigators to enrich HSCs, further differentiating these cells from precursors. In this study anti-CD34, anti-CD135 and anti-CD117 were used to analyse and separate the HSC populations by FACS. The results obtained were; 29% of CD34+ cells, 8% of CD135+ cells and 64% of CD117+ cells. We clearly identified that using a pre-enrichment step which utilises the positive selection procedure by magnetic nanobeads with the anti-CD117 antibody (c-Kit) potentiates the isolation of the true HSC population which can be easily sorted by FACS. We were able to isolate and sort approximately 446654 HSCs using this anti-cKit methodology from 6 x 106 cells (approximately 1% of the total bone marrow isolates). According to the literature this is a very accurate approximation of true HSCs in the bone marrow of normal young adult mice. Our data emphasise that for appropriate transplantation and engraftment of HSCs, it is recommended to use our procedure for isolation and purification in order to enhance the efficacy of the transplantation. Our data strongly suggest that using more than one step to isolate HSCs will increase the yield of bona fide stem cells and will facilitate transplantation.
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| 653 |
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|a تحاليل الدم
|a توصيف المنتجة للدم
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| 700 |
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|9 44002
|a Petratos, Steven
|e Advisor
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| 856 |
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|u 9843-001-004-0012-T.pdf
|y صفحة العنوان
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| 856 |
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|u 9843-001-004-0012-A.pdf
|y المستخلص
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| 856 |
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|u 9843-001-004-0012-C.pdf
|y قائمة المحتويات
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| 856 |
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|u 9843-001-004-0012-F.pdf
|y 24 صفحة الأولى
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| 856 |
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|u 9843-001-004-0012-1.pdf
|y 1 الفصل
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| 856 |
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|u 9843-001-004-0012-2.pdf
|y 2 الفصل
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| 856 |
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|u 9843-001-004-0012-3.pdf
|y 3 الفصل
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| 856 |
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|u 9843-001-004-0012-4.pdf
|y 4 الفصل
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| 856 |
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|u 9843-001-004-0012-5.pdf
|y 5 الفصل
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| 856 |
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|u 9843-001-004-0012-R.pdf
|y المصادر والمراجع
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| 930 |
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|d n
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| 995 |
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|a +Dissertations
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| 999 |
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|c 620119
|d 620119
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