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Effect medium volume and explants density on in vitro shoot formation and growth of pineapple " Ananas comosus L. Mer " cv. Moris

المصدر: المجلة الليبية العالمية
الناشر: جامعة بنغازي - كلية التربية بالمرج
المؤلف الرئيسي: Hamad, Abdelhamid M. (Author)
المجلد/العدد: ع15
محكمة: نعم
الدولة: ليبيا
التاريخ الميلادي: 2017
التاريخ الهجري: 1438
الشهر: مارس
الصفحات: 1 - 10
DOI: 10.37376/1570-000-015-010
ISSN: 2518-5845
رقم MD: 763033
نوع المحتوى: بحوث ومقالات
اللغة: الإنجليزية
قواعد المعلومات: EduSearch
مواضيع:
كلمات المؤلف المفتاحية:
Medium volume | Explants density | Pineapple | Total shoots | Ananas comosus
رابط المحتوى:
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024 |3 10.37376/1570-000-015-010 
041 |a eng 
044 |b ليبيا 
100 |9 402648  |a Hamad, Abdelhamid M.  |e Author 
245 |a Effect medium volume and explants density on in vitro shoot formation and growth of pineapple " Ananas comosus L. Mer " cv. Moris 
260 |b جامعة بنغازي - كلية التربية بالمرج  |c 2017  |g مارس  |m 1438 
300 |a 1 - 10 
336 |a بحوث ومقالات  |b Article 
520 |b  The effect of combinations of four explants density (one, two, three and four explants) and ten volumes (3, 4, 5, 6, 7, 8, 9, 10, 11, 12 ml) of full strength liquid MS medium enriched with sucrose at 20 g/l and 6-benzyalaminopurine (BAP) at 2.0 mg/l on the Moris pineapple in vitro shoot formation rate, shoot length and expected total shoots per one liter of medium were investigated. The results showed that a difference of 2 to 5 ml in the medium volume per culture could cause a loss of 50 % of the possibly obtainable rate of shoot formation. At density of one explant per culture, 10 ml of medium resulted in formation of 13 shoots while 4 ml of medium resulted in formation of 6 shoots per explant. At density of two, three and four explants, using of 11, 9 and 10 ml of medium per culture resulted in formation of 10, 11 and 7 shoots per explant respectively while using of 6, 7 and 5 ml of medium per culture resulted in formation of 5, 5 and 3 shoots per explant. Overall, the shoots formation declined from 9 at density of one to 4 shoots per explant at density of four. The medium volume per culture had no independent significant effect on shoot formation per explant but interacted significantly with explants density. Lower explants density (one explants) and larger medium volume (10 ml) was best treatment for shoot formation per explants (13 shoots). However, the highest total shoots (7500 shoots) per liter of medium obtained using three explants and dispensing the medium at smallest volume (4 ml) per culture.  
653 |a الحرب العالمية الثانية   |a الأطباء الفرنسيون   |a بوكاي، موريس  |a البحوث العلمية  
692 |b Medium volume  |b Explants density  |b Pineapple  |b Total shoots  |b Ananas comosus 
773 |4 العلوم الإنسانية ، متعددة التخصصات  |4 العلوم الاجتماعية ، متعددة التخصصات  |6 Humanities, Multidisciplinary  |6 Social Sciences, Interdisciplinary  |c 010  |e Global Libyan Journal  |f Al-Mağallaẗ al-libiyyaẗ al-ʿalāmiyyaẗ  |l 015  |m ع15  |o 1570  |s المجلة الليبية العالمية  |v 000  |x 2518-5845 
856 |u 1570-000-015-010.pdf 
930 |d y  |p y  |q y 
995 |a EduSearch 
999 |c 763033  |d 763033