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الرئيسية > Somatic Short Tandem Repeat ST...>الوصف

Somatic Short Tandem Repeat STR DNA Profiling of Hand Washed Detergent Treated and Bleached Blood Stains

العنوان بلغة أخرى: تأثير بعض المنظفات الكيميائية وطرق غسيل بقع الدماء الجافة على البصمة الوراثية الجسدية
المصدر: المجلة الجنائية القومية
الناشر: المركز القومي للبحوث الاجتماعية والجنائية
المؤلف الرئيسي: حسن، مجدي (مؤلف)
مؤلفين آخرين: Al Rashidy, Saif A. (Co-Author) , Refaat, Ahmed M. (Co-Author) , Choudhry, Abdul Rauf (Co-Author) , Bin Dukhyil, Abdul Aziz A. (Co-Author)
المجلد/العدد: مج58, ع2
محكمة: نعم
الدولة: مصر
التاريخ الميلادي: 2015
الشهر: يوليو
الصفحات: 168 - 180
ISSN: 0028-0054
رقم MD: 798208
نوع المحتوى: بحوث ومقالات
اللغة: الإنجليزية
قواعد المعلومات: IslamicInfo
مواضيع:
المنظفات الكيميائية | البصمة الوراثية | الإثبات الجنائي
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المستخلص: DNA evidence, linking perpetrators to crime scenes, is crucial to many legal proceedings. Blood is one of the most common physical evidence in investigations of violent crimes. Forensic analysis of the blood found at a crime scene supply in many ways valuable information that can be decisive in solving of a crime. Blood stains can be found anywhere a violent crime was committed . Blood stain patterns on the floor (from a dripping wound, for example) or spattered on the walls can be interpreted for crime scene reconstruction. DNA typing analysis can establish the genetic profile(s) of the participant (s) in a violent crime. Consequently, blood stains are among the most useful evidence for court. This fact is becoming well-established so that criminals now often attempt to clean up their crime scene. Blood samples were obtained from 5 unrelated donors and the substrates used in this study were pieces of white Saudi uniforms cloth (30% cotton and 70% silk), these pieces of cloth impregnate with blood were treated by different ways of hand washing including only water, soap compound (Arial), chlorinated bleach (Clorox), in addition soap and chlorinated bleach together. DNA from blood stains were extracted and quantified. Autosomal STR genotyping of DNA isolated from hand -washed, detergent- treated and bleached blood stains were size separated on 3130 Genetic Analyzer. Blood stain washed by water only were not affected. In the majority of samples complete autosomal STR profile were obtained from water or and from detergent washed blood stains whereas bleach-washing of the blood stains resulted in the loss of 2-3 autosomal STR loci. Blood stain washed with detergent and Clorox together, produced partial human DNA profile. Autosomal STR loci D7S820 CSFIPO and D18551 appeared to the most be affected mostly by bleach and or detergent washing.
ISSN: 0028-0054

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