المستخلص: |
Smooth cayenne pineapple explants were cultured on full strength MS medium solidified with agar at 7 g/l, enriched with sucrose at 30 g/l and Benzylaminopurine (BAP) at 2.25 mg/l and subcultured every 60 days on the same but fresh medium for eight consecutive subcultures. Shoots formation per explant increased over the first five subcultures from 7 at the first to 16 shoots at the fifth then declined to 8 shoots over the next three subcultures. Total length and total weight of shoots per explant increased from 72 mm and 1.59 g at the first to reach a maximum of 149 mm and 2,58 g by the fifth subculture. Average length and average weight of shoot, on the contrary, decreased over subcultures from 11 mm and 0.24 g at the first to 7 mm and 0.09 g by the fifth subculture. However, the shoot resumed gaining weight and length after that to reach by the eighth subculture equal length and weight as that of the first subculture. Assuming that all of the in vitro produced shoots at each subculture were used on the next subculture, biomass weighting 25 tons and a total of 103 millions shoot could be produced after eight subcultures (16 months).
|