Detection of Microdeletions in the AZF Region of the Y-chromosome in Azospermic Palestinian Males
| العنوان بلغة أخرى: |
الكشف عن طفرات (AZF) على كرموسوم Y عند الرجال الفلسطينين العديمي الحيوانات المنوية |
|---|---|
| المؤلف الرئيسي: | Taha, Shaden (Author) |
| مؤلفين آخرين: | Rappocciolo, Emilia (Advisor) |
| التاريخ الميلادي: |
2016
|
| موقع: | بيرزيت |
| الصفحات: | 1 - 46 |
| رقم MD: | 865147 |
| نوع المحتوى: | رسائل جامعية |
| اللغة: | الإنجليزية |
| الدرجة العلمية: | رسالة ماجستير |
| الجامعة: | جامعة بيرزيت |
| الكلية: | كلية الدراسات العليا |
| الدولة: | فلسطين |
| قواعد المعلومات: | Dissertations |
| مواضيع: | |
| رابط المحتوى: |
عدد مرات التحميل
2
| المستخلص: |
Infertility is a global concern that affects approximately 10-15% of couples. Over 30 million males worldwide are infertile-up to 30% of which are azospermic. Y chromosome microdeletions are one of the most common genetic causes of non-obstructive azospermia. The aim of this study was to screen a group of infertile males from the West Bank of Palestine to identify the frequency of Y microdeletions and assess the significance of this testing for intracytoplasmic sperm injection candidates. DNA extracted from blood samples of 41 azospermic males, 50 normospermic males and 2 females were analyzed by PCR to detect 17 sequence tagged sites (STS) along the long arm of the Y chromosome. The primers were specific to the Azospermia Factor (AZF) regions, AZFa, AZFb and AZFc, where a missing STS indicated a microdeletion. The results showed that 4/41 cases (~9.8%) were deleted in seven different AZFb and AZFc locations, whereas no microdeletions were found in any of the fertile control samples. Results indicated that the frequency in the Palestinian population is similar to that of other world populations and suggests the need for AZF screening in Palestine, in particular for azospermic males undergoing testicular sperm retrieval for assisted reproduction. |
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