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In Vitro and in Vivo Inhibition of Gammarus Pulex Acetylcholinesterase by Diazinon

المصدر: المجلة العلمية لكلية التربية
الناشر: جامعة مصراتة - كلية التربية
المؤلف الرئيسي: Elwahaishi, Salem (Author)
مؤلفين آخرين: Mohamed, Wesam F. A. (Co-Author) , Hargreaves, Alan (Co-Author) , Mills, Chris Lloyd (Co-Author)
المجلد/العدد: س5, ع12
محكمة: نعم
الدولة: ليبيا
التاريخ الميلادي: 2019
الشهر: مارس
الصفحات: 14 - 27
رقم MD: 1007708
نوع المحتوى: بحوث ومقالات
اللغة: الإنجليزية
قواعد المعلومات: EduSearch
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المستخلص: This study was designed to investigate the effect of the in vivo and in vitro exposure to the organo phosphorothioate pesticide diazinon, its metabolite diazinon oxon and diazinon in a commercial formulation on the AChE activity of the freshwater amphipod Gammarus pulex. Only diazinon oxon at concentrations of ≥ 50x103 nM oxon caused a significant inhibition of acetylcholinesterase (AChE) activity in vitro. However, diazinon oxon had no significant impact on the enzyme activity following in vivo exposure. The latter mode of exposure to either ≥ 10 nM of diazinon or ≥ 5 nM of the diazinon in the commercial formulation caused a significant reduction in AChE activity of treated organisms. This study supports the finding of previous studies that suggested AChE activity in aquatic animals as a valuable biomarker of diazinon.

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