المستخلص: |
The fungal community associated with different species of grains infected with grain moth (Sitotroga cerealella) was studied. Fifty-nine fungal isolates belonging to 19 fungal species (13 genera) and sterile mycelia were isolated from infected Sorghum grains with Grain Moth (Sitotroga cerealella) after storage for 4 months. The lowest number of fungal species and isolates were 24 isolates belonging to 8 fungal species and sterile mycelia after 8 months of storage. Statical analysis using X2 showed a significant difference between isolates numbers and storage periods. From infected wheat grains with grain moth, (Sitotroga cerealella) 40 fungal isolates belonging to 12 species were isolated after 2 months of storage. The total fungal isolates after 4 and 8 months were 33 and 24 isolates, respectively. And there was no significant differences between isolates numbers and storage periods. From dead insects on Sorghum, 33 fungal isolates belonging to 12 species were isolated after storage for 2 months, while 24 isolates belonging to 12 fungal species were isolated from dead insects on wheat grains after 8 months of storage. The isolates numbers of fungi from both Wheat and Sorghum grains increased due to the increasing of insects numbers infected these grains, the total numbers of isolates from Sorghum and Wheat grains when infected with 20 pairs of moth insects were 30 after 2 weeks and 37 after 1 month, respectively. There were differences between the total numbers of isolates, numbers of fungal species and genera isolated from different species of grains at the same storage period and a same level of insect numbers. The highest number of fungal and isolates were 15 and 36 respectively on Wheat grains, while the lowest were on barley grains after 2 months. Most isolated fungi in this study were belonging to Deuteromycotina, followed by Zygomycotina. Aspergillus species had the highest frequencies. Similarity values, Sorenson's index (I) and Coefficient of association (V) showed a strong association between fungal species isolated from Wheat grains and insects, and between fungal species isolated from Sorghum grains and insects. This study showed that 30 isolates of Aspergillus flavus had the ability to produce B1, B2 and other aflatoxins.
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