Molecular Identification of Ten Maize Genotypes using EST-SSR Markers
| المصدر: | مجلة جامعة الحسين بن طلال للبحوث |
|---|---|
| الناشر: | جامعة الحسين بن طلال - عمادة البحث العلمي والدراسات العليا |
| المؤلف الرئيسي: | Al-Saadi, Taif Razzaq Majeed Najar (Author) |
| مؤلفين آخرين: | Al-Tamimi, Attyaf Jameel Thamir (Co-Author) |
| المجلد/العدد: | مج5, ملحق |
| محكمة: | نعم |
| الدولة: |
الأردن |
| التاريخ الميلادي: |
2019
|
| الصفحات: | 337 - 347 |
| DOI: |
10.36621/0397-005-985-021 |
| ISSN: |
2519-7436 |
| رقم MD: | 1035927 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | EduSearch, AraBase, HumanIndex |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
EST-SSRs | Fingerprint | Maize
|
| رابط المحتوى: |
عدد مرات التحميل
1
| المستخلص: |
Molecular identification of ten maize (Zea mays L.) genotypes was accomplished using ten of Express Sequence Tag-Simple Sequence Repeats markers ( EST-SSRs) .Molecular size of amplified fragments ranged 114bp to315bp in primers p-umc1566 and p-bnlg 1016 respectively. Primer p-bnlg 1016 gave highest values for genotype number., allele number., gen diversity and polymorphic information content (PIC).Highest value for major allele frequency produced by p-bnlg1767 and GRMZM2G098290 while highest heterozygosity produced by p-umc2189. The most and least efficient primers were 1 (GRMZM2G098290) and p-umc2189 respectively. Highest amplified allele was in p-umc2189 ,unique allele in primers (p-umc2189, p-bnlg1016 and p-bnlg1017) ,polymorphic allele in primers p-bnlg1016 and 1(GRMZM2G098290).Primer p-bnlg1016 gave highest value for number of unique fingerprint .Phylogenetic tree divided maize genotypes in two main clusters the first was small included only Sarah and Buhooth 106 genotypes , while the other large one included eight genotypes which further divided in to two subclusters. |
|---|---|
| ISSN: |
2519-7436 |
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