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Phytochemical, Antioxidant and Antimicrobial Activites of Boscia Senegalensis Seed

المؤلف الرئيسي: Omer, Mnahil Suliman (Author)
مؤلفين آخرين: Hamdi, Omer Abdalla Ahmed (Advisor)
التاريخ الميلادي: 2019
موقع: الخرطوم
الصفحات: 1 - 46
رقم MD: 1104507
نوع المحتوى: رسائل جامعية
اللغة: الإنجليزية
الدرجة العلمية: رسالة ماجستير
الجامعة: جامعة النيلين
الكلية: كلية الدراسات العليا
الدولة: السودان
قواعد المعلومات: Dissertations
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المستخلص: This study was conducted with the aim to extract and analyze the seeds powder of Boscia senegalensis, which collected from forest on the north of Alfasher in Darfour state, Sudan. Two methods of extractions from the seeds powder of B.seneglensis were performed ;method 1: extraction by maceration using three different solvents including methanol, water and ethyl acetate their yields were 29%, 9.6% and 17.3%, respectively. These extracts were subjected to phytochemical analysis which showed the presence of saponins, coumarins, carbohydrates, tannins, flavonoids and alkaloids. Method 2: successive extractions using soxhlet extractor from seeds powder using n hexane, chloroform and methanol yielded 4.7%, 4.1% and 15.7 %, respectively. GC MS was used for quality and quantity evaluation of the oil extract of seeds revealed the presence of a total of 14 compounds were completely identified and the major compounds were classified as unsaturated fatty acid including Tetratiacontane (22.71%),. Hexaatriacontare (18.41%), Linolenic acid (16.75%) Hexadecanoic acid (11.85), Oleic acid (7.14%) and Squalene (5.16%). Anti-oxidant activity was evaluated for all extracts. The results showed that the methanol extract (mac) possessed the highest activity with 76.7% at the concentration of 0.1 mg/ ml using DPPH assay. The most active extract was subjected to TLC and visualized with vanillin –HSO₄ reagent, afforded nine pure compounds were analyzed by spectroscopic methods (Infra-red and Ultra-Violt).