| المستخلص: |
Background: Airborne microbes play an important role in indoor and outdoor air quality. The study aimed to investigate the enumeration and identification of bacteria and fungi in bio aerosols at various parts of a chicken feedlot in Irbid-Jordan and their relationship to some important meteorological parameters. Methods: This study was conducted on a case study of one chicken feedlot in the northeast of Irbid during the Oct-Nov months in the autumn season. Samples were separated into four groups based on the age of the chickens. Moreover, air samples were collected from indoor at the ground, first, and second floor and outdoor at 5m, 25m, and 50m using passive sampling method, water and faeces sample using a sterile container. Then the observation colonies were counted by culture-based method and expressed as Colony Forming Unit (CFU). Temperature, relative humidity, wind speed, wind direction, and ultraviolet index (UV) were also measured at the time of sampling. Results: The bacteria identified belonged to the genera of Proteus, E. coli, Klebsiella, Staphylococcus, Streptococcus, Citrobacter, Pseudomonas and Enterobacter spp., while Aspergillum is the only isolated genus among the fungi. The mean total number of the microbial count at all stages in indoor air samples was 211±61.72 CFU/1m2/h and the most dominant bacterial genus included E. coli (35.2%), Proteus (28.7%), and Klebsiella (16.9%) respectively, which come from animal sources. In the outdoor air, the mean total number of the microbial count at all stages was 48± 9.62 CFU/1m2/h and the most dominant bacterial genera were Staphylococcus (34.0%), Streptococcus (28.8%) and E. coli (12.2%). The mean total number of fungi in the indoor and outdoor air samples were 8±2.39 and 10±2.75 CFU/1m2/h respectively. Also, there was a significant relationship between the degree of microbial contamination and meteorological parameters (P<0.05). The total bacterial count in all water samples was exceeded the acceptable limit (1,000 CFU/mL). Conclusion: According to the results, the number of bacteria in both air samples and water increases with the age of the chicken. While fungi were affected by the outdoor air of the farm (source of contamination). Also, there was a significant relationship between the degrees of microbial contamination and meteorological parameters (P<0.05).
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