Resazurinreagent to Determine the Safe Dose of PPAR-γ Agonist for Reducing Activated Gliosis Using 3T3 Cells
| المصدر: | المجلة الجامعة |
|---|---|
| الناشر: | جامعة الزاوية - مركز البحوث والدراسات العليا |
| المؤلف الرئيسي: | Abushofa, Fikry A. (Author) |
| المجلد/العدد: | مج18, ع3 |
| محكمة: | نعم |
| الدولة: |
ليبيا |
| التاريخ الميلادي: |
2016
|
| الشهر: | أغسطس |
| الصفحات: | 18 - 24 |
| رقم MD: | 1263808 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | EduSearch, EcoLink, IslamicInfo, AraBase, HumanIndex |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
In Vitro Alternative | Spectrophotometric Analysis | Resazurin | Confluent | 3T3
|
| رابط المحتوى: |
الناشر لهذه المادة لم يسمح بإتاحتها. |
| المستخلص: |
A method is described spectrophotometric test, for the assessment of the possible toxicity effects of the agent, such as PPAR-γ agonist (rosiglitazone) on 3T3 cells in culture. The last three decades of the nineteenth century the 3T3 became very common use in biology that easy to become confluent. These cells are sensitive and reproducible assay lend themselves to a screening procedure of potential toxicants which can help reduce the use of animals for toxicity testing. The PPAR-γ agonist such as rosiglitazone will be used in further experiments to reduce activated gliosis and inhibits astrocyte proliferation in a mouse model which represents the neurodegenerative disorder. The purpose of the assay is to verify that the reason for reducing the astrocytes is due to the positive effect of the PPAR-γ agonist not for its toxicity. The results were analysed by a FLUOstar Galaxy software reader, Results showed that Rosiglitazone was non-toxic at the concentrations used in this assay. |
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