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الرئيسية > علاقة فعالية أنزيم بولى أمين أ...>الوصف

علاقة فعالية أنزيم بولى أمين أوكسيديز ومركبات متعدد الأمين فى دم النساء المصابات بداء السكر وتأثير بعض مركبات الثايويوريا المحضرة مختبرياً على مستوى الفعالية ومستوى السكر فى الفئران المصابة بداء السكر التجريبى

المؤلف الرئيسي: اللهيبي، نشوان إبراهيم عبو شاهين (مؤلف)
مؤلفين آخرين: آل فليح، خولة أحمد محمود (مشرف), حميد، أياد سعدي (مشرف)
التاريخ الميلادي: 2013
موقع: تكريت
التاريخ الهجري: 1434
الصفحات: 1 - 160
رقم MD: 615243
نوع المحتوى: رسائل جامعية
اللغة: العربية
الدرجة العلمية: رسالة دكتوراه
الجامعة: جامعة تكريت
الكلية: كلية التربية
الدولة: العراق
قواعد المعلومات: Dissertations
مواضيع:
الكيمياء الحياتية | أنزيم بولى أمين أوكسيديز | مرضى السكري | الإناث | الفحوصات الطبية
رابط المحتوى:
صفحة العنوان     
المستخلص     
قائمة المحتويات     
24 صفحة الأولى     

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المستخلص: This Study included determination of polyamine oxidase (PAO) activity level in female diabetic patients type I, II and non treated diabetic females. It was found that the activities of PAO in red blood cells (RBC) and plasma in above patients were significantly higher than that of normal(p<0.05). Plasma glucose, glycosylated hemoglobin and malondialdehyed in RBC and plasma were determined, and found significantly higher in female diabetic patients type I,II and non treated diabetic females than that of normal (p<0.05). According the correlation factor(r) direct relationships were found between PAO and these parameters. At the same time the glutathione, vitamin C and E were determined in RBC and plasma of type I, II and non treated diabetic females and found to be significantly lower than that of normal(p<0.05), with inverse relationship between PAO and these parameters . In cases above, plasma urea and creatinine were also determined, the results indicated a higher (but non significant levels) than that of normal (p>0.05) and with no relationships with PAO according to (r). Results of polyamine (PA) compounds analysed by using higher performance liquid chromatography technique indicated that the level of PAs in RBC was higher than that of plasma and spermine level was higher than that of spermidine and diaminopropane levels.

Partial purification of PAO from RBC of diabetic and normal females were included in this study. This was achieved by using dialysis, ion-exchange chromatography and SDS-PAGE electrophoresis respectively. Three proteinous peaks with PAO activities in RBC (I,II,III) from each of normal, diabetic with specific activities Umg protein (0.194,0.182 and 0.098), (0.349, 0.237 and 0.176) and (0.453, 0.615 and 0.47) respectively with molecular weight (72588, 74512 and 69339), (141458, 100671 and 104018) and (139620, 130779 and 100015) KD respectively. Also three proteinous peaks have PAO activity were obtained by purification of non-treatment diabetic females RBC with sp.A (0.317, 0.255 and 0.243) Umg protein and with M.wet (106080, 104018 and 130779) KD respectively. These results indicated the presence of PAO at least as three isoenzymes with different molecular weight in diabetic patients as compared to normal. This study didn't show the existence of Cu2+ ion as a cofactor for any PAO isoenzymes, but indicated the existence of FAD as a cofactor for all PAO isoenzymes. On the other hand this study was included a preparation of thiourea derivatives of structure analogous to sulfonylurea drugs and containing semicarbazide, thiosemicarbazide or hexylamine. Semicarbazide and thiosemicarbazide showed inhibitory effect on partially purified PAO activity. The prepared thiourea derivatives of semicarbazide and thiosemicarbazide showed competitive inhibition of PAO activity while the prepared thiourea derivatives of hexylamine showed uncompetitive inhibition of PAO activity . Alloxan diabetic mice was treated orally by the prepared thiourea derivatives with dose (7.14)mgKg body weight daily for 21 days. All prepared thiourea derivatives showed significant lowering effect in plasma glucose and also PAO acivity levels in plasma and RBC. The more effective derivatives was N[(Benzoylamino)]thioxymethyl-semicarbazide (B) since it showed a more lowering effect on plasma glucose level to (7.11)mmolL in diabetic mice after 21 day. The lethal dose LD50 of this compound was determined and found to be 428.57 mgkg body weight.

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