المستخلص: |
The adrenal gland of amphibians is composed of a mixed population of adrenochromaffin and corticosteroid-secreting cells. To localize chromaffin cells, previous immunohistochemical studies conducted on adrenal gland have used antisera against the enzymes implicated in the biosynthesis of cathecolamines such as anti-tyrosine hydroxylase, anti-phenylethanolamine-N-metyltransferase, or dopamine-B- hydroxylase. In the present study, we demonstrate by immunofluorescence procedure the localization of frog adrenal chromaffin cells using a monoclonal antibody directed against dopamine substance . In addition, our immunohistochemical study confirms the co- localization of* dopamine and adrenaline in adrenal chromaffin cells.
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