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Analysis of the diversity of the sclerotinia in Australia and search for Gemini like mycoviruses

المؤلف الرئيسي: Al Harbi, Riyadh Awad (Author)
التاريخ الميلادي: 2010
موقع: بريزبن
الصفحات: 1 - 30
رقم MD: 752957
نوع المحتوى: رسائل جامعية
اللغة: الإنجليزية
الدرجة العلمية: رسالة ماجستير
الجامعة: Queensland University
الكلية: Science College
الدولة: أستراليا
قواعد المعلومات: +Dissertations
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المستخلص: Mycoviruses are capable of infecting fungi and can help to control fungal crop due to its ability to confer hypovirulence factors. Mycoviruses generally possess double or single stranded-stranded RNA genomes, with few DNA mycoviruses being previously characterized. This report discusses the hypovirulence associated with a DNA mycovirus that is able to be conferred to the plant pathogen, Sclerotinia sclerotiorum. This particular DNA mycovirus is known as Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1, or SsHADV-1, which is 2166 nucleotides in size. SsHADV-1 possesses genetic material coding for REP which is (a replication initiation protein) and CP which is (a coat protein). S. sclerotiorum DNA has been amplified as well as cloned successfully. The extracted DNA from the Sclerotinia has been showed bands above 2000 bp and that means they are S. sclerotiorum. With the discovery of a single stranded DNA mycovirus that helps confer hypovirulence factors to plant pathogenic S. sclerotiorum, we are one step closer in preventing unacceptable crop loss, which is a global phenomenon that contributes to food security issues.

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