Characterization of Extracellular Polymeric Substances "EPS" Extracted from Pseudomonas Putida ATCC 11172 Growths at Different Carbon Concentration
| المصدر: | مجلة العلوم والدراسات الإنسانية |
|---|---|
| الناشر: | جامعة بنغازي - كلية الآداب والعلوم بالمرج |
| المؤلف الرئيسي: | El Ayatt, Abu Baker K. (Author) |
| مؤلفين آخرين: | Gonzalez, Maria E. Romero (Co-Author) |
| المجلد/العدد: | ع19 |
| محكمة: | نعم |
| الدولة: |
ليبيا |
| التاريخ الميلادي: |
2016
|
| الشهر: | نوفمبر |
| الصفحات: | 1 - 14 |
| DOI: |
10.37376/1571-000-019-003 |
| ISSN: |
2312-4962 |
| رقم MD: | 763343 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | HumanIndex |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
Extra Cellular Polymeric Substances "EPS" | Pseudomonas Putida ATCC 11172 | Ethylene Diamine Tetra Acetic Acid "EDTA" | 2-Keto-3 Deoxyoctonate "KDO" | Uronic Acid
|
| رابط المحتوى: |
عدد مرات التحميل
8
| المستخلص: |
The extracellular polymeric substances (EPS) extracted from Pseudomonas putida ATCC 11172 at different carbon concentration, P. putida was grown using Luria broth alone (LB) and supplemented with glucose at 0.5% w/v (LBG 0.5%) and 1.0% w/v (LBG 1%) in order to promote different EPS composition.. The Bound EPS were extracted by ethylene diamine tetra acetic acid (EDTA) methods and precipitated by cold ethanol 3:1; while free EPS extracted only by precipitate by cold ethanol 3:1. Study the effect of carbon concentration on the Protein and polysaccharides contains. Where found the extracted EPS had carbohydrate/ protein ratio between 1.18-1.62; indicating the EPS extracted is pure. Low and stable values of 2-keto -3 deoxyoctonate (KDO) that meaning no significant cell rupture occurred during the extraction of EPS, the amount of Uronic acid was relatively high and similar in all EPS samples, this is indicative that the composition of EPS has only low contamination by intercellular material. |
|---|---|
| ISSN: |
2312-4962 |
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