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Photosynthetic bacteria are favorable candidates for biological hydrogen production due to their high conversion efficiency and versatility in the substrates they can utilize. Rhodobacter sphaeroids NCMB 8253 a Photosynthetic non-sulfur bacterium was used to study the growth and its ability to produce hydrogen from organic compounds under anaerobic conditions. The kinetics and the effect of various parameters on growth of Rhodobacter sphaeroides NCIMB 8253 were investigated in 1L screw capped bottle containing 112 medium under anaerobic condition with light source. The culture was incubated at 30°C for 78 hours with different pHs. Result shows the optimum pH about 6.5 with specific growth at 0.0312 h-1 and doubling time 22.21 h. The result show ability of bacterium to produce hydrogen at optimum pH and 30°C at certain period Hydrogen production; Photosynthetic bacteria; Rhodobacter sphaeroides; pH effect Biological hydrogen production from residual organic substrates with phototrophic microorganisms, in connection with wastewater treatment, is a promising option. Photo biological production of hydrogen can be performed by photoautotrophic or photoheterotrophic organisms. The basic principles photo biological hydrogen production Microalgae and cyanobacteria are photoautotrophic organisms because they can use light as the energy source and carbon dioxide as sole carbon source. Some bacteria are termed photoheterotrophic microorganisms because in spite of their ability of using light as the energy source, they need organic carbon as the carbon source. Photosynthetic bacteria, cyan-bacteria, algae or fermentative bacteria are commonly utilized for biological hydrogen production (Nandi & Sengupta, 1998; Das & Veziroglu, 2001). Among these, the photosynthetic bacteria are favorable candidates for large-scale production due to their high substrate conversion e6ciencies and their capability of using a wide variety of substrates either for growth or hydrogen production.
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