المستخلص: |
The present study aimed to investigate the in vitro antimicrobial activity and phytochemical screening of rhizome and leaves extracts of Turmeric (Curcuma longa. L) plant. Curcuma longa rhizome and leaves extracts were prepared using sequential extraction method by petroleum ether (PE) and methanol: chloroform (M:C) (1:1) solvents. Antimicrobial activity was investigated against four standard bacteria (Bacillussubtilus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli) and two fungi (Aspergillusniger and Candida albicans) using paper disc diffusion method. Phytochemical examinations were implemented for all plant extracts using different standard methods. The results revealed that, M:C extracts of Curcuma longa rhizome and leaves showed the presence of flavonoids, phenols, steroids and terpenoids, whereas PE extracts of rhizome and leaves showed the presence of steroids and terpenoids only. Highest antimicrobial activity was measured against S. aureus by PE extract of rhizome, while PE extract of leaves showed moderate antimicrobial activity against B.subtillus. At the same time, M:C extract of rhizome recorded strongest antibacterial activity against B.subtillus, whereas the highest effect against E.coli was recorded by M:C extract of leaves. PE extract of rhizome at 20 and 30 mg/ml showed antibacterial activity against S. aureus more than that of Ampicillin/Sulbactam 20 μg/disc, Ciprofloxacin 5 μg/disc and Gintamicin10 μg /disc whereas, M:C extract of leaves at 30mg/ml displayed higher antibacterial activity than that of Gintamicin10 μg /disc against E.coli. In conclusion, PE and M:C extracts of Curcuma longa rhizome and leaves could be exploited as a good source and alternative natural antibacterial agents especially against S. aureus, B. subtillusand E.coli.
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