Sterilization and establishment of pineapple shoot tip
| المصدر: | المجلة الليبية العالمية |
|---|---|
| الناشر: | جامعة بنغازي - كلية التربية بالمرج |
| المؤلف الرئيسي: | Hamad, Abdelhamid M. (Author) |
| المجلد/العدد: | ع16 |
| محكمة: | نعم |
| الدولة: |
ليبيا |
| التاريخ الميلادي: |
2017
|
| التاريخ الهجري: | 1438 |
| الشهر: | ابريل |
| الصفحات: | 1 - 9 |
| DOI: |
10.37376/1570-000-016-002 |
| ISSN: |
2518-5845 |
| رقم MD: | 763038 |
| نوع المحتوى: | بحوث ومقالات |
| اللغة: | الإنجليزية |
| قواعد المعلومات: | EduSearch |
| مواضيع: | |
| كلمات المؤلف المفتاحية: |
BAP | Ananas comosus | Sterilization | Establishment | Contamination | Clorox
|
| رابط المحتوى: |
عدد مرات التحميل
7
| المستخلص: |
Out of eight sterilization treatments made of combinations of Mercuric chloride (HgCl2), Clorox and Ethanol at different concentrations and exposure times, Clorox (25%) for 25 minutes was the best treatment resulting in successful sterilization of 56 % of crown tips of Smooth cayenne pineapple. The sterilized crown tip grew and produced multiple shoots on agar solidified full strength MS medium enriched with 6-benzyleaminopurine (BAP) at four different concentrations (2.25, 3.25, 4.25 and 5.25 mg l-1), but at different shoot formation rate. After 60 days of incubation, the highest shoot formation (7 shoots / tip) obtained in medium enriched with BAP at 2.25 mg l-1 followed by BAP at 3.25 mg l-1 (4 shoots). BAP at 4.25 and at 5.25 mg l-1 resulted in formation of 3 and 2 shoots per tip. The low percentage of successfull sterilization of crown tips (56 %) indicated that elimination of microbial ccontamination is still a serios problem and main drawback that prevent full investigation and optimization of establishment of crown tips of pineapple. Nevertheless, the few successfully sterilized crown tip proliferated into shoots good enough to investigate the next multiplication stage. |
|---|---|
| ISSN: |
2518-5845 |
عناصر مشابهة
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Biweekly Changes In The In Vitro Shoot Formation and Growth Pattern of Pineapple ( Ananas Comosus L Merr ) Cv Smooth Cayenne Over 105 Days of Incubation on Different Hormone Treatments
بواسطة: Hamad, Abdelhamid M. منشور: (2017) -
Effect of Media Type, Hormone Treatments and Incubation Periods on The In Vitro Shoot Formation and Growth of Pineapple ( Ananas Comosus L Merr ) Cv. Smooth Cayenne
بواسطة: Hamad, Abdelhamid M. منشور: (2017) -
Massive in Vitro Biomass and Propagules Production of Pineapple: Ananas Comosus L.Merr) cv Smooth Cayenne
بواسطة: Hamad, Abdelhamid M. منشور: (2017) -
Effect of Peatmoss and Sand Mixing Ratio on the Acclimatization of Smooth Cayenne Pineapple: Ananas comosus "L" Merr
بواسطة: Hamad, Abdelhamid M. منشور: (2016) -
Effect of Explants Length and Segmentation and Separated and Cluster of Shoots on the in Vitro Shoot Formation and Growth of Pineapple: Ananas comosus L. Merr
بواسطة: Hamad, Abdelhamid M. منشور: (2017)
