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|3 10.37376/1570-000-016-002
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|a eng
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|b ليبيا
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|9 402648
|a Hamad, Abdelhamid M.
|e Author
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|a Sterilization and establishment of pineapple shoot tip
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260 |
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|b جامعة بنغازي - كلية التربية بالمرج
|c 2017
|g ابريل
|m 1438
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|a 1 - 9
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336 |
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|a بحوث ومقالات
|b Article
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|b Out of eight sterilization treatments made of combinations of Mercuric chloride (HgCl2), Clorox and Ethanol at different concentrations and exposure times, Clorox (25%) for 25 minutes was the best treatment resulting in successful sterilization of 56 % of crown tips of Smooth cayenne pineapple. The sterilized crown tip grew and produced multiple shoots on agar solidified full strength MS medium enriched with 6-benzyleaminopurine (BAP) at four different concentrations (2.25, 3.25, 4.25 and 5.25 mg l-1), but at different shoot formation rate. After 60 days of incubation, the highest shoot formation (7 shoots / tip) obtained in medium enriched with BAP at 2.25 mg l-1 followed by BAP at 3.25 mg l-1 (4 shoots). BAP at 4.25 and at 5.25 mg l-1 resulted in formation of 3 and 2 shoots per tip. The low percentage of successfull sterilization of crown tips (56 %) indicated that elimination of microbial ccontamination is still a serios problem and main drawback that prevent full investigation and optimization of establishment of crown tips of pineapple. Nevertheless, the few successfully sterilized crown tip proliferated into shoots good enough to investigate the next multiplication stage.
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|a الأناناس
|a التعقيم
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692 |
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|b BAP
|b Ananas comosus
|b Sterilization
|b Establishment
|b Contamination
|b Clorox
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773 |
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|4 العلوم الإنسانية ، متعددة التخصصات
|4 العلوم الاجتماعية ، متعددة التخصصات
|6 Humanities, Multidisciplinary
|6 Social Sciences, Interdisciplinary
|c 002
|e Global Libyan Journal
|f Al-Mağallaẗ al-libiyyaẗ al-ʿalāmiyyaẗ
|l 016
|m ع16
|o 1570
|s المجلة الليبية العالمية
|v 000
|x 2518-5845
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856 |
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|u 1570-000-016-002.pdf
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|d y
|p y
|q y
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|a EduSearch
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|c 763038
|d 763038
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