| المستخلص: |
The anti tumor activity of a 15-day treatment of Nigella sativa L. (NS) against (MCYST-LR) was tested in mice induced by using 3 groups (10 each). T1 group was treated i.p. (intraperitoneally) with 25 μg/kg b.w. MCYST-LR. T2 mice were administrated orally with 800mg/kg b.w. of NS prior to the injection intraperitoneally with Mcyst-LR (25μg/kg b.w). C mice were injected only with DMSO considered as a control group. Since, the change on the sequences of DNA, gives an indication on the accuracy of the treatment (i.e. before and after treatment), so Biochemical and genetic toxicity effects will be detected. In case of T1 and T2, the number and size of metastases in liver was used as indicator for tumorgensis. T1 showed a significantly increased in Alkaline phosphatase (ALP) in plasma (P<0.05), but decreased in T2. Plasma transaminases level (ALT, AST) and (LDH) decreased significantly in T2 as compared to controls (C) (P<0.001), while showed a significant increased in T1. The concentration of GGT, LPO in T1 were significantly increased, and its values near to the control in T2. Antioxidants such as lipid peroxidases (LPO), ceruloplasmin (CP), superoxidase dismutase (SOD) and total thiols were significantly decreased in the plasma of T1 compared to (C), while they increased in T2. A significant increase (p<0.001) in TBARS, SOD and total glutathione (GSH) was estimated in liver tissues of T1 compared to (C) while these parameters were significantly decreased in T2 and were close to the values of (C). Flurometric data analysis showed a significant increase in DNA damage in T1 compared to T2 and control groups. In conclusion, NS oil decreased the amount of MCYST-LR in plasma and liver compared to MCYST-LR only treated mice. Our results demonstrate that NS has an important effect as a tumor supressor against MCYST-LR. It endowed with antitumor action, which displays a potent by stander effect validating further exploration of its applicability in human cancer gene therapy.
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